In order to assess the contribution of downstream signaling components of the PI3K pathway to GBM cell migration, T98G cells were transiently transfected with siRNAs targeting the three Akt isoforms (Akt1-3), the ribosomal protein S6 kinase (S6K1), or the small GTP-binding protein Rac1. The gene discussed is PIK3CA; the disease is glioblastoma.