U2OS cells strongly overexpressed MMP9 (152-fold increase), confirming the high metastatic potential of this cell line coupled with high expression levels of genes involved in cell cycle regulation (i.e., BRCA1, CCNE1, CDC25A, CHEK2, E2F1), apoptosis (i.e., BCL2, BCL2L1), membrane integrity and tumor invasion (i.e., COL18A1, ITGA3, MMP9, MCAM), cell proliferation (i.e.,. The gene discussed is CHEK2; the disease is neoplasm.