An approach for detection of cardiac injury, tissue ischemia and myocardial infarction involves measurements of the well known cardiac marker enzymes for example, creatine kinase (CK), cardiac creatine kinase-MB fraction (CK-MB), AST, ALT, ALP, LDH, and cholesterol in serum [29,30]. The gene discussed is GPT; the disease is myocardial infarction.