NOB1 and infection: Fig. 1B and C show that, after 96 h of incubation, infection in Saos-2 and U2OS cells was highly efficient (>80%). Knockdown efficiency was determined using qPCR, which showed that the relative levels of NOB1 mRNA transcripts were significantly decreased by almost 50% in the Lv-shNOB1 group as compared with those in the Lv-shCon and Con groups in the two cell lines (Fig. 1D and E). These results demonstrated that endogenous NOB1 expression was specifically inhibited by the Lv-shNOB1 construct.