In our present study, analyses of the DNA methylation status of MUC1, MUC2 and MUC4 in pancreatic juices were useful for differential diagnosis of human pancreatic neoplasms i.e. PDAC, intestinal-type IPMN and gastric-type IPMN, with high specificity and sensitivity. The gene discussed is MUC4; the disease is pancreatic neoplasm.