To test if MNKs regulate expansion of MM cells via their phosphorylation of eIF-4E, we stably transfected wild type (WT), a serine-to-alanine (SA) mutant (at S209) or serine-to-aspartic acid (ASP) phosphomimetic eIF-4E mutant into ANBL-6 and 8226 MM cells. Here, EIF4E is linked to Miyoshi myopathy.