Hence, we analyzed cell surface KIAA1114 expression on short-term passages of KIAA1114high and KIAA1114low subpopulations sorted from HuH7 and SK-Hep-1 cell lines by flow cytometry to analyze whether KIAA1114high HCC cells could produce KIAA1114low cells and vice versa. Here, TRO is linked to hepatocellular carcinoma.