In summary, our study characterized KIAA1114 as a novel cell-surface marker of liver TICs using human HCC cell lines and showed that isolated KIAA1114high fractions from two different HCC subsets (AFP+ and AFP−) displayed subtype-specific TIC features, resembling those of EpCAM+ TICs in AFP+ HCC and CD90+ TICs in AFP− HCC. The gene discussed is AFP; the disease is hepatocellular carcinoma.