As both estriol and progesterone were capable of controlling hyperglycemia in the alloxan induced mice model for T1DM, and as the pancreatic β cells were destroyed by the diabetogenic compound, the anti diabetic effect of these steroids cannot be mediated through the pancreatic synthesis of insulin, an essential hormone in glucose metabolism, and the steroids were capable of inducing insulin synthesis in the liver cells not only in vivo, but both estriol and progesterone was found to be efficiently synthesized insulin due to the steroids induced NO synthesis in vitro liver homogenate as well. This evidence concerns the gene INS and type 1 diabetes mellitus.