As reported before the liver cells from alloxan treated mice were capable of synthesizing insulin (12) to determine the role of the hepatic insulin synthesis in the control of hyperglycemia in alloxan treated mice, when the liver cell homogenate was prepared as described in the Methods, and treated with different concentrations of estriol, it was found the maximal NO was achieved at 3.5 μM (Fig. 3). Here, INS is linked to Hyperglycemia.