In summary, this study demonstrated that PRO2000/ANCCA was overexpressed in HCC and was associated with clinicopathological features such as number of tumor nodules, TNM stage, tumor microsatellite, portal vein tumor thrombus and recurrence, but not with gender, age, cirrhosis, HBV infection and serum AFP level. This evidence concerns the gene ATAD2 and hepatocellular carcinoma.