Although combination of Angiotensin-II infusion and unilateral nephrectomy are able to replicate many features of injury observed in the db RAS, the db RAS model is likely more physiologically relevant to the development of diabetic nephropathy in patients with both diabetes and RAS, and will allow the development of mechanistic studies to identify critical pathways related to inflammation, fibrosis, oxidative stress, and cell cycle regulation that are responsible for the development and progression of diabetic renal disease. This evidence concerns the gene AGT and diabetic kidney disease.