In contrast to hypoglycemia, hyperglycemic exposure for 24 h did not affect the ZO-1 overall expression (Figure 2B), although disruption of ZO-1 band at cell-cell contacts (see white arrows) and a slight increase in total ZO-1 expression was observed following the initial 3 h treatment, compared to control (Figure 2A). This evidence concerns the gene TJP1 and Hypoglycemia.