The primary findings of this study are that the absence of CLIC4 results in smaller kidneys with fewer glomeruli and less dense peritubular capillary network, increased proteinuria that is primarily albumin with no increase in β-2 microglobulinuria, and increased susceptibility to the acute kidney injury induced by folic acid with no difference in the functional or histologic recovery from acute injury. Here, CLIC4 is linked to urogenital neoplasm.