ALCAM and neoplasm: In the multivariable analysis, tumor regression grade, ypN, CD166 and p21 expression levels in pre-operative biopsy samples were significantly associated with disease free survival, showing that a high expression level of CD166 and p21 exhibited shorter disease free survival (HR 5.614, p = 0.003 in CD166, HR 6.146, p = 0.001 in p21, Table 3, Figure 2).