To further detect the preventive effect of SFN on T2DM-induced aortic fibrosis, immunohistochemical staining showed the increased expression of profibrotic mediators, CTGF (Figure 3(a)), and TGF-β1 (Figure 3(b)), in aortic tunica media of diabetic mice. Here, TGFB1 is linked to type 2 diabetes mellitus.