In 1989 Shiel and Jason [38] reported that patients referred to a community rheumatologist because of a positive ANA with a titer of 1/40 or greater (comprising 8.8% of all referrals compared to 4.1% in our study) received a specific diagnosis in 86.6% and of those, 51.4% had a connective tissue disease [38]. This evidence concerns the gene BTG3 and connective tissue disorder.