To determine whether the 3′UTR of PRRX1 mRNA is a functional target of miR-214 in CRC cells, the target sequence of PRRX1 3′UTR (wt 3′UTR) or the mutant sequence (mt 3′UTR) were cloned into a luciferase reporter vector (Fig.3E). This evidence concerns the gene PRRX1 and colorectal carcinoma.