AMH and Tay-Sachs disease: This first report of Sox9 gene silencing by RNAi in a gonad culture system of turtles displaying TSD shows a coordinated expression of both genes, supporting the hypothesis that SOX9 directly or indirectly regulates Amh. Although possible off-target effects and the viability of the transfected organs remain to be further investigated, the demonstrated efficacy of this technique in turtle gonadal cultures expands our toolbox to address critical questions of sex determination pathways in TSD.