DERL3 and colorectal cancer: On transfection of DERL3 in the hypermethylated HCT-116 colorectal cancer cell line, the cells proved to be significantly less proliferative in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Fig. 2a), and had a significantly lower percentage colony formation density (Fig. 2b) than empty vector (pIRES2-eGFP)-transfected cells.