Furthermore, we have demonstrated that inhibition of the Shh signaling pathway significantly inhibited EMT by suppressing the activation of transcription factors Snail and Slug, which were correlated with significantly reduced pancreatic cancer stem cell invasion [26], [27], [47], [49], [50], suggesting that the Shh signaling pathway is involved in early metastasis. This evidence concerns the gene SHH and familial pancreatic carcinoma.