The purpose of the present study was to investigate the effect of BTX-A on CGRP and SP levels in the jugular plasma and medulla oblongata containing caudal trigeminal nucleus of the rat model of migraine induced by nitroglycerin (NTG), and then to determine, in a randomized controlled trail, whether acupoint-sites injection of BTX-A is effective in the treatment of migraine and to compare its efficacy with that obtained by fixed-sites injection of BTX-A, which is one of the most commonly used methods of BTX-A for migraine therapy in patients. The gene discussed is TFF2; the disease is migraine disorder.