BCP and pre-S sequences were obtained from HBV DNA positive individuals including 97 HCC patients (86 males, 11 females), 72 LC patients (56 males, 16 females), 80 CH patients (59 males, 21 females), and 79 controls (36 males, 43 females). The gene discussed is OPN1SW; the disease is laryngotracheoesophageal cleft.