KDM1A and colorectal neoplasm: It has been shown previously that de novo DNA methylation cannot take place on nucleosomes, in which H3K4me2 or H3K4me3 occur.48 A recent study has demonstrated overexpression of LSD1 in colorectal tumors.49 Therefore, we treated our cells with an inhibitor of LSD150 to preserve H3K4me2 levels, and thereby prevent DNA methylation.