The present study also examined Nrf2 and Nrf2-related gene GCLC and GCLM expression.Nrf2 is a basic leucine zipper transcription factor and plays important roles in mediating cellular antioxidant mechanisms that rescue the liver from a wide variety of toxicants [27], [47]–[49].Accumulating evidence suggests that the Nrf2-ARE pathway exerts diverse biological functions against a variety of liver injury [50], and is proposed to be a potential therapeutic target for liver fibrosis [51]. Here, NFE2L2 is linked to Hepatic fibrosis.