GPC3 is highly expressed in HCC, while be less frequent in preneoplastic or entirely absent in normal liver tissue [36]–[38].It was reported that the frequency of GPC3 expression in AFP-negative HCC patients is as high as 90% [39].This has made GPC3 as an useful and specific tumor diagnostic marker in the diagnosis for HCC. The gene discussed is AFP; the disease is hepatocellular carcinoma.