Histopathological results showed that ethanol + DEN treatment can aggravate hepatocytes lesions in progressive liver fibrosis, cirrhosis, hepatocytes atypical hyperplasia and the formation of abnormal fission, even changes in the structure of the liver, and eventually leaded to hepatocarcinogenesis.GPC3 is an useful early diagnostic markers for HCC, it's immunohistochemical results also suggested the formation of HCC. This evidence concerns the gene GPC3 and Hepatic fibrosis.