On the contrary, subjects with NCI and AD showing the interaction of TDP-43 with p65 in coimmunoprecipitation experiments (Figure 1b,c; Subjects 7, 14, 21 and 29; NCI-p and Subject 2; AD-p) and MCI without the interaction of TDP-43 with p65 (Figure 1b,c; MCI-n) presented only few cells expressing p65 in the nucleus (Figures 3b-d, Additional file 1: Figure S1c). This evidence concerns the gene TARDBP and Alzheimer disease.