In the present study, we found that the strongly positive rate of LETM1 protein was significantly higher in poorly differentiated HNSCC than in well and moderately differentiated cases (P = 0.016), and it was also higher in HNSCC with lymph node metastasis than in cases with nonmetastasis (P = 0.001). The gene discussed is LETM1; the disease is head and neck squamous cell carcinoma.