In the present study, we found that the strongly positive rate of LETM1 protein was significantly higher in poorly differentiated HNSCC than in well and moderately differentiated cases (P = 0.016), and it was also higher in HNSCC with lymph node metastasis than in cases with nonmetastasis (P = 0.001). Here, LETM1 is linked to metastatic malignant neoplasm in the lymph nodes.