Univariate analysis demonstrated that tumor differentiation (P = 0.005), TNM stage (P = 0.002), chemoradiotherapy (P = 0.019), and LETM1 expression status were significantly associated with 5-year disease-free survival and overall survival in patients with HNSCC (Table 3). The gene discussed is LETM1; the disease is head and neck squamous cell carcinoma.