GPT and metabolic dysfunction-associated steatotic liver disease: By means of the present HFD fed rat model, we have been able to demonstrate most of the clinical aspects of NAFLD in humans such as increased hepatic lipids accompanied by different degrees of steatosis histologically, elevated serum ALT, increase in body weight and adiposity, elevated serum FFA, increased HOMA-IR index, and elevated hepatic lipid peroxides level.