As previously observed in male CD1 mice (Figure 1), male offC PVH Irs2, Glut3, Kir6.2, and Crh mRNA expression was down-regulated in response to insulin-induced hypoglycemia (offC sal vs offC hypo); however, no such change occurred in female offC, despite identical states of severe hypoglycemia in all groups (<2.5 mM, Figure 5, A and B). The gene discussed is IRS2; the disease is Hypoglycemia.