IRS2 and Hypoglycemia: A strong response to glycemic state was apparent, particularly in the PVH: expression of glucose transporter 3 (Glut3), insulin receptor substrate 2 (Irs2), the KATP-channel subunit Kir6.2, and corticotrophin releasing hormone (Crh) were down-regulated during hypoglycemia 60 minutes after insulin injection (Figure 1A), suggesting rapid glucose-sensitive PVH transcriptome regulation.