The number of activated (CD25+ CD62Llow) and IFNγ-producing OTII CD4 T cells was significantly greater in the 12-month-old CR mice than 12-month-old ad libitum mice (AL-LRD) and was comparable with young 2-month-old mice (2MO) (Fig. 5), verifying the findings from the tumor setting. The gene discussed is CD4; the disease is neoplasm.