CD8A and neoplasm: Cytotoxic activity was measured based on surface expression of the degranulation marker CD107a on CD8 T cells (gating strategy shown in Supplementary Figure S1).29 We observed an induction in CD107a-positive CD8 T cells when pre-treatment T cells were exposed to treatment-affected tumor cells as opposed to pre-treatment tumor cells (P<0.05) (Figure 1a).