To test the ability of these TCRs to recognize the endogenous SSX2 expressed by tumor cells, we cultured PBLs (transduced with TCR-5, -8, -9, -11) with cell lines derived from melanoma (888, SKMEL-23, 624), glioma (U251), and breast cancer (MCF-7). This evidence concerns the gene SSX2 and central nervous system cancer.