SOX9 and prostate carcinoma: To validate the modulation of NWD1 expression by SRY-related factors, HEK 293T and primary prostatic carcinoma (PPC-1) cell lines were transfected with constructs overexpressing the prototypical SRY (Sex-determining region Y protein) or the family counterpart SOX9 (SRY-Related HMG-Box, Gene 9) and then subjected to quantitative PCR analysis.