On day 14, the mildly anemic TC-1 mice had mildly elevated liver hepcidin mRNA levels as compared to controls (>2×, P = 0.012) (Figure 3B), while the more severely anemic LLC mice had lower hepcidin levels (P = 0.022), perhaps because of the counterregulatory effect on hepcidin of moderate anemia and the resulting erythropoietic stimulus [28]. This evidence concerns the gene HAMP and anemia.