GLB1 and infection: When 1294 was added to cultures at the timepoint where HFF infection with N. caninum tachyzoites was initiated, and incubated for 2 hours before removal by washing, there was a significant decrease in beta-galactosidase activity compared to experiments where compound 1266 [16] (negative, low-potency control) or DMSO were assessed (t-test; p<0.001) (Figure 3A).