Of the 48 HCC samples, 20 (41.7%) showed high Nkx2.8 expression (Nkx2.8++ or Nkx2.8+++), and the remaining 28 (58.3%) displayed low Nkx2.8 expression (Nkx2.8- or Nkx2.8+) (Figure 2, Table 1). The gene discussed is NKX2-8; the disease is hepatocellular carcinoma.