To evaluate the alteration of stemness gene expression during the process by which As2O3 induced differentiation of HCC CSCs, we analyzed stemness gene expression by means of a Human Stem Cell RT2 Profiler PCR Array after the CD133+ Huh7-wt cells had been treated with 4 μM As2O3 for 48 hours. Here, PROM1 is linked to hepatocellular carcinoma.