EGFR and neoplasm: Such exon 9 PIK3CA mutations were discussed not to have an independent effect on anti-EGFR efficacy [8] and in all three patients we found the same mutation also in pretreatment samples, i.e. primary tumor (#2 and #5) or metastasis (#8) (Table S1) suggesting that targeted deep sequencing has not contributed to the identification of therapy related changes in the tumor genomes of our patient cohort.