Overall, Rb1 treatment reduced average food intake by 11% (p<0.05) in HF diet fed mice, and a reduction of food intake was observed on days 7, 8, 10, 12, 14, 19 and 20 of Rb1 treatment (all p<0.05, Fig. 1A) compared with HF control group. This evidence concerns the gene RB1 and hydrops fetalis.