However, no differences were found for histone markers around the -132 site between normal and cancer tissues in BRCA1 wild type cases (Supplementary Fig. S3).These results, together with the methylation data in Figs. 1, 2, and 3, suggest that PEMT transcription may be associated with changes in epigenetic features, including reduced GCN5-related H3K9ac enrichment and increased EHMT-1-related H3K9me enrichment around the hypermethylated -132 site in BRCA1-mutated breast cancer. The gene discussed is BRCA1; the disease is cancer.