In agreement with these considerations, diagnostic criteria for acute HHEV infection among patients with acute hepatitis would be as follows: (1) IgM negative, RNA positive (window period); (2) IgM positive, RNA positive (early seroconversion stage); (3) IgM positive, RNA negative (post-seroconversion stage); and (4) Seroconversion to IgG antibody on follow-up. Here, CD40LG is linked to Acute hepatitis.