RHOT1 and neuroblastoma: Importantly, FCCP-induced ubiquitination of endogenous Miro1/2 could also be observed in neuroblastoma cells overexpressing YFPParkin (Fig. 2D) and was dependent on PINK1 because ubiquitination levels were greatly reduced in dopaminergic SH-SY5Y cells expressing shRNA to PINK1 compared with nonsilencing shRNA-expressing cells (Fig. 2, E and F).