The current study also showed the expansion of splenic CD4+ and CD8+ T cells during the course of P. berghei infection although the phenotypes of T cells infiltration in the brain differed between the co-infected and P. berghei-mono-infected mice; CD8+ T cells were lower in co-infected mice when compared to P. berghei-mono-infected mice, and increasing the worm load during the co-infection further turned down the levels of CD8+ T cells. This evidence concerns the gene CD4 and coinfection.