Hepatic collagen deposition, as evidenced by Sirius Red staining, and α-SMA-positive activated HSCs (4.3 ± 0.9 vs. 20.3 ± 3.8 cells/hmf; P < 0.005) were also more evident in AnxA1 KO mice with NASH. This evidence concerns the gene ANXA1 and metabolic dysfunction-associated steatohepatitis.