Furthermore, within 27 samples failed in FISH, 25 samples have successful IHC, of the remained 2 one IHC 3+ bronchial biopsy tissue failed to FISH assess shorting for FISH enumeration due to loss of tumor cells in deeper procedure was confirmed on following transthoracic rebiopsy tissue showing IHC 3+ and FISH positive; another one IHC 2+ ectomy tissue failed to assess the ALK FISH due to loss of fluorescence signal, but was confirmed positive by PT-PCR at the consecutive-cut TWS. The gene discussed is ALK; the disease is neoplasm.