In this study, we evaluated ALK status using manually semiquantitatively IHC and FISH in a cohort of 410 unselected adenocarcinomas, aiming to get the epidemiological data of ALK positive in lung adenocarcinoma patients and demonstrate that ALK D5F3 IHC correlates well with FISH in tissue whole sections. The gene discussed is ALK; the disease is lung adenocarcinoma.