WT1 overexpression by itself is an independent and negative indicator for predicting CR rate, DFS and OS of the CN-AML patients; moreover, it increases the statistical power of predicting the same clinical outcomes when it is combined with the NPM1mt or the FLT3ITD genotypes that are the good or poor prognostic markers of CN-AML. The gene discussed is WT1; the disease is acute myeloid leukemia.