Figure 1A depicts the levels of DSC3 transcripts across four independent published microarray studies [19], [20], [21], [22]. To experimentally corroborate this observation, we tested a panel of prostate cell lines including LNCaP, DU145, 22RV1, benign cells namely RWPE and PrEC, by Q-PCR. In a pattern consistent with microarray data the benign cells showed higher transcripts expression when compared with cancer cell lines (Figure 1B). Similar expression pattern was observed for GSTP1, a well-known methylated gene in prostate cancer which we used as a positive control (Figure 1C). This evidence concerns the gene GSTP1 and prostate cancer.