Furthermore, to validate the binding of HIF-1α with CypA promoter, through the binding of HIF-1α to the CypA promoter in pancreatic cancer cell line BXPC3, an independent ChIP assay was performed and the promoter fragment was specifically co-immunoprecipitate by anti- HIF-1α, so we proved that HIF-1α could bind directly to the hormone response element sites in the CypA promoter area and regulate CypA expression. This evidence concerns the gene PPIA and familial pancreatic carcinoma.