AHNAK and neoplasm: To verify the function of Ahnak in tumor-suppressive activity, we crossed mice carrying the mammary gland specifically expressed polyomavirus middle T antigen (MMTV-PyVT) with Ahnak−/− mice,23 and MMTVTg/+Ahnak−/− and MMTVTg/+Ahnak+/+ mice were generated from mating of female MMTVTg/+Ahnak+/− mice with male MMTV+/+Ahnak+/− mice (Figure 6a).