To assess whether antibody engagement of CD81 promotes actin polymerization and alters hepatoma morphology, Huh-7.5 cells were transfected to express AcGFP.CD81 and Ruby tagged LifeAct, a fluorophore labeled peptide that binds F-actin allowing live cell imaging of the actin cytoskeleton [21]. Here, CD81 is linked to hepatocellular carcinoma.