JAG1 and ovarian cancer: To verify whether the upregulation of JAG1 was attributed to the loss of miR-199b-5p in ovarian cancer, both in situ hybridization (ISH) and immunohistochemistry (IHC) were performed to examine the expression level of miR-199b-5p and JAG1, respectively, on a commercial human ovarian cancer tissue array (OVC1021, Pantonmics).