To know whether the expression of PLA2R1 might be regulated by DNA methylation, we treated VHL-positive (ACHN) and -negative (RCC10 and 786-O) derived RCC cell lines with the 5-azadeoxycytidine (5-aza-dC) DNA methyl transferase (DNMT) inhibitor. The gene discussed is DNMT1; the disease is renal cell carcinoma.